proteinLength

291

308

geneLength

876

924

Biological materials

Mutant

GP1283 (''[[gene|citA]]''::''aphA3''), available in [SW|Jörg Stülke]'s lab

GP1753 (''[[gene|citR]] [[gene|citA]]''::''aphA3''), the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

GP2360 (''[[gene|citR]] [[gene|citA]]''::''erm''), the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

BKE09430 (Δ[[gene|citR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA, downstream forward: _UP4_TAGGAGCAACCAAAACGCCT

BKK09430 (Δ[[gene|citR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA, downstream forward: _UP4_TAGGAGCAACCAAAACGCCT

GP1283 (''[[gene|citA]]''::''aphA3''), available in [SW|Jörg Stülke]'s lab

GP1753 (''[[gene|citR]] [[gene|citA]]''::''aphA3''), the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

GP2360 (''[[gene|citR]] [[gene|citA]]''::''erm''), the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

Biological materials

Expression vectors

pGP2264 (N-terminal His-tag, purification from ''E. coli'', in [SW|pETM-11]), available in [SW|Jörg Stülke]'s lab

pGP1023 (C-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP574]), available in [SW|Jörg Stülke]'s lab

pGP1029 (N-terminal Strep-tag, purification from ''B. subtilis'', in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

pGP1030 (C-terminal Strep-tag, purification from ''B. subtilis'', in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

pGP2260 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

pGP2262 (integration into [[gene|ganA]], expression under the control of the xylose-inducible PxylA promoter in ''B. subtilis'', in [SW|pGP888]), available in [SW|Jörg Stülke]'s lab

_ec

Biological materials

Expression vector

pGP2264 (N-terminal His-tag, purification from ''E. coli'', in [SW|pETM-11]), available in [SW|Jörg Stülke]'s lab

pGP1023 (C-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP574]), available in [SW|Jörg Stülke]'s lab

pGP1029 (N-terminal Strep-tag, purification from ''B. subtilis'', in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

pGP1030 (C-terminal Strep-tag, purification from ''B. subtilis'', in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

pGP2260 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

pGP2262 (integration into [[gene|ganA]], expression under the control of the xylose-inducible PxylA promoter in ''B. subtilis'', in [SW|pGP888]), available in [SW|Jörg Stülke]'s lab